Data items in the EXPTL_CRYSTAL_GROW_COMP category record
details about the components of the solutions that were 'mixed'
(by whatever means) to produce the crystal.
In general, solution 1 is the solution that contains the
molecule to be crystallized and solution 2 is the solution
that contains the precipitant. However, the number of solutions
required to describe the crystallization protocol is not limited
Details of the crystallization protocol should be given in
_exptl_crystal_grow_comp.details using the solutions
described in EXPTL_CRYSTAL_GROW_COMP.
Example 1 - based on PDB entry 5HVP and laboratory records for the
structure corresponding to PDB entry 5HVP.
1 1 1 'HIV-1 protease' '0.002 ml' '6 mg/ml'
; The protein solution was in a buffer containing 25 mM NaCl,
100 mM NaMES/ MES buffer, pH 7.5, 3 mM NaAzide
1 2 2 'NaCl' '0.200 ml' '4 M' 'in 3 mM NaAzide'
1 3 2 'Acetic Acid' '0.047 ml' '100 mM' 'in 3 mM NaAzide'
1 4 2 'Na Acetate' '0.053 ml' '100 mM'
; in 3 mM NaAzide. Buffer components were mixed to produce a
pH of 4.7 according to a ratio calculated from the pKa. The
actual pH of solution 2 was not measured.
1 5 2 'water' '0.700 ml' 'neat' 'in 3 mM NaAzide'
Key Category Items
Items in Category exptl_crystal_grow_comp