An Information Portal to Biological Macromolecular Structures
PDB Home |		Dictionary Resources Home  |  Dictionary  |  Category Groups  |  Categories  |  Items  |  Data

Item _em_electron_diffraction.details

Description

   Details of the electron diffraction experiment

Category

Item Examples

Example 1:

 THE MODEL WAS DERIVED USING ELECTRON DIFFRACTION
  AND IMAGE DATA FROM TWO DIMENSIONAL CRYSTALS OF TUBULIN
  INDUCED BY THE PRESENCE OF ZN++ IONS.
  WHAT FOLLOWS ARE THE COORDINATES FOR THE AB-TUBULIN DIMER
  BOUND TO TAXOL AS OBTAINED BY ELECTRON CRYSTALLOGRAPHY OF
  ZINC-INDUCED SHEETS.  THIS IS THE UNREFINED MODEL, BUILT
  INTO A RAW DENSITY MAP WHERE THE RESOLUTION IN THE PLANE
  OF THE SHEET WAS 3.7 ANGSTROMS AND THAT PERPENDICULAR TO
  THE SHEET ABOUT 4.8 ANGSTROMS.  THE MODEL DOES NOT CONTAIN
  MOST OF THE C-TERMINAL RESIDUES OF EITHER MONOMER WHICH
  WERE DISORDERED IN THE MAP.  THE LOOP BETWEEN HELIX H1 AND
  STRAND S2, AND THAT BETWEEN H2 AND S3 ARE PRESENT FOR
  COMPLETENESS BUT WERE BUILT INTO VERY WEAK DENSITY.
  GIVEN THE LIMITED RESOLUTION OF THE MAP, THE CONFORMATION
  OF THE SIDE CHAINS, ESPECIALLY THOSE CORRESPONDING TO
  RESIDUES ON THE SURFACE OF THE DIMER, MUST BE TAKEN
  CAUTIOUSLY.  IN ADDITION, BECAUSE THIS IS AN UNREFINED
  MODEL, CERTAIN GEOMETRY ERRORS MAY STILL BE PRESENT IN THE
  STRUCTURE.  PLEASE TAKE THIS INTO ACCOUNT WHEN
  INTERPRETING YOUR OWN DATA BASED ON THE PRESENT TUBULIN
  STRUCTURE.  ALTHOUGH THE POSITION OF RESIDUES (WITH THE
  EXCEPTION OF THOSE IN THE LOOPS MENTIONED ABOVE) SHOULD
  NOT CHANGE SIGNIFICANTLY UPON REFINEMENT, DRAWING
  INFORMATION AT THE LEVEL OF SIDE CHAIN CONFORMATION IS
  CLEARLY NOT ADVISED.  FINALLY, PLEASE NOTICE THAT THE
  TAXOID IN THE MODEL IS THE TAXOL DERIVATIVE TAXOTERE.

1

Mandatory Code

Data Type Code

 

© RCSB PDB