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An Information Portal to Biological Macromolecular Structures |
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Category pdbx_entity_src_gen_prod_pcr 
Category Description This category contains details for the PCR steps used in the overall protein production process. The PCR is assumed to be applied to the result of the previous production step, or the gene source if this is the first production step.
Category Examples 
Example 1:
Example 1 - hypothetical example
_pdbx_entity_src_gen_prod_pcr.entry_id 111000111
_pdbx_entity_src_gen_prod_pcr.entity_id 222000111
_pdbx_entity_src_gen_prod_pcr.step_id 1
_pdbx_entity_src_gen_prod_pcr.next_step_id 2
_pdbx_entity_src_gen_prod_pcr.end_construct_id 440050000111
_pdbx_entity_src_gen_prod_pcr.robot_id 5
_pdbx_entity_src_gen_prod_pcr.date '2002-07-12:15:13'
_pdbx_entity_src_gen_prod_pcr.forward_primer_id 2
_pdbx_entity_src_gen_prod_pcr.reverse_primer_id 3
_pdbx_entity_src_gen_prod_pcr.reaction_details
; Annealing temperature = 70 C
Annealing time = 60 s
Extending temperature = 74 C
Extending time = 120 s
Melting temperature = 95 C
Melting time = 120 s
Number of cycles = 40
Polymerase = KOD
Template = 10 pmol
Primer = 25 pmol
Total volume = 25 ul
;
_pdbx_entity_src_gen_prod_pcr.purification_details
; No purification
;
Key Category Items 
Items in Category pdbx_entity_src_gen_prod_pcr