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Category em_vitrification

Category Description

 Data items in the EM_VITRIFICATION category
  record details about the method and cryogen used in
  rapid freezing of the sample on the grid prior to its
  insertion in the electron microscope




Category Examples




Example 1:

 Example 1 - based on PDB entry 1DYL and laboratory records for the
                structure corresponding to PDB entry 1DYL




 
  _em_vitrification.entry_id               1DYL
  _em_vitrification.id                     1
  _em_vitrification.sample_preparation_id  1
  _em_vitrification.cryogen_name           "ETHANE"
  _em_vitrification.humidity               90
  _em_vitrification.temp                   95
  _em_vitrification.instrument             .
  _em_vitrification.method                 "PLUNGE VITRIFICATION"
  _em_vitrification.time_resolved_state    .
  _em_vitrification.citation_id            1
  _em_vitrification.details                
  ; SAMPLES WERE PREPARED AS THIN             
    LAYERS OF VITREOUS ICE AND                
    MAINTAINED AT NEAR LIQUID NITROGEN        
    TEMPERATURE IN THE ELECTRON MICROSCOPE    
    WITH A GATAN 626-0300 CRYOTRANSFER        
    HOLDER.                            
  ;




Key Category Items

• _em_vitrification.id
• _em_vitrification.entry_id



Items in Category em_vitrification

Item Name	Mandatory Code
_em_vitrification.citation_id	no
_em_vitrification.cryogen_name	no
_em_vitrification.details	no
_em_vitrification.entry_id	yes
_em_vitrification.humidity	no
_em_vitrification.id	yes
_em_vitrification.instrument	no
_em_vitrification.method	no
_em_vitrification.sample_preparation_id	no
_em_vitrification.temp	no
_em_vitrification.time_resolved_state	no

 

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