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Item _em_particle_selection.details

Description

  General details on the particle picking method

Category

Item Examples

Example 1:

  Particles were picked using a program written in-house for selecting 
   filaments. Lines are drawn over the filaments using the computer mouse, 
   and the program selects coordinates for boxing the particles at a 
   user-selected interval while keeping track of groups of particles 
   coming from the same filament. This is useful where polarity can be 
   determined per filament by two-dimensional averaging. The program also 
   keeps track of the angle of the overlaid, user-drawn line, which is 
   used for preliminary vertical orientation of the particles. 
   8440 particles of 80 x 80 pixel size were selected from the filaments. 

1

Example 2:

  A reference was established by selecting a well preserved particle 
   and symmetrizing it 20-fold rotationally. Cross-correlation functions 
   of this reference with images of digitized micrographs containing 
   adsorbed particles of PM28 revealed correlation peaks at the particle 
   positions, irrespective of their angular orientation. Using this 
   particle picking method a gallery of 4096 particles was created. 

2

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